Quality level: 100
- sufficient for ≤1,250 reactions (12032945001)
- sufficient for ≤2,500 reactions (12032953001)
- sufficient for ≤250 reactions (12032929001)
- sufficient for ≤50 reactions (12032902001)
- sufficient for ≤500 reactions (12032937001)
- 1000 U pack (12032937001 4×250 U)
- 2500U pack (12032945001 10x250U)
- 5,000 U package (12032953001 20×250 U)
- 100 U pack (12032902001)
- 500 U pack (12032929001 2×250 U)
Mfr. no.: Roche
Parameter: 72 ° C optimum reaction temperature.
Sent in: dry ice
FastStart ™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 ° C but is activated by a 2-4 minute heat activation step at +95 ° C. Since it is inactive at low temperatures, FastStart ™ Taq DNA polymerase cannot elongate nonspecific primer-template hybrids that can form at those temperatures. FastStart ™ Taq DNA Polymerase is an ideal tool for hot-start PCR because the enzyme remains inactive during PCR setup and prior to the initial denaturation step.
FastStart ™ Taq DNA Polymerase is a chemically modified, thermostable form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to + 25 ° C during PCR setup and then activated at + 95 ° C during initial denaturation. This enzyme offers superior results due to its unique enzyme design and optimized damping system. FastStart Taq DNA Polymerase is an ideal tool for hot-start PCR because the enzyme remains inactive during the PCR setup and before the initial denaturation step. It can be requested for:
- Multiplex PCR
- Difficult templates, for example, secondary structures or GC-rich sequences
- Automated PCR, e.g. room temperature handling
- Hot start PCR up to 3 kb
- Hot start RT-PCR up to 3 kb
- Quantitative Reverse Transcription PCR (RT-qPCR)
- Bisulfite specific PCR
Use FastStart ™ Taq DNA Polymerase, a DNA pack with a ready-to-use PCR nucleotide mix. For added convenience, select ready-to-use FastStart ™ PCR Master Concentrate 2 ×.
Features and Benefits
FastStart Taq DNA polymerase is a modified recombinant Taq DNA polymerase, inactive at temperatures below + 75 ° C. The kit includes an optimized PCR buffer and a GC-RICH solution to manipulate a wide range of templates. High enzyme stability allows pipetting using robotic stations.
- Higher specificity, sensitivity and performance:
The hot-start PCR makes it easy to set up the PCR.
- Use the robotic setup.
Use this stable enzyme mix for 24 hours at +15 to + 25 ° C.
- Avoid contamination by PCR carry-over.
Incorporate dUTP and use uracil-DNA glycosylase to pretreat PCR master mixes
- optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution to handle a wide range of templates
- Superior results thanks to its unique enzyme design and optimized damping system.
1 kit containing 5 components
The function of each lot is tested using human genomic DNA and primers specific for the 365 bp fragment of the human tPA gene and a 284 bp fragment of the Apo E gene with a GC content of 74%. Each lot is also tested for the absence of Exo and endonucleases and cleavage activity.
Definition of unit
1 μg of M13mp9ss DNA, 0.3 μg of the M13 sequencing primer and 0.1 μCi of α-32P dCTP are incubated with varying amounts of FastStart Taq DNA polymerase units in 50 μl of incubation buffer at +65 ° C for 60 min. The amount of incorporated dNTPs is determined by precipitation with trichloroacetic acid.
Unit Assay: 1 μg of M13mp9ss DNA, 0.3 μg of M13 Sequencing Primer, and 0.1 μCi of α-32P dCTP are incubated with varying amounts of FastStart Taq DNA polymerase units in 50 μl of incubation buffer. at +65 ° C for 60 min. The amount of incorporated dNTPs is determined by precipitation with trichloroacetic acid.
Volume activity: 5 U / μl
Storage Conditions (Working Solution): Fast Start Taq Master Mix is stable for 4 weeks at -25 ° C to -15 ° C without primers
Please Note: this parameter is not part of the specification
For life science research only. It should not be used in diagnostic procedures.